【摘要】 经测定鼠尾采血获取的血清,10只SCID鼠在所设实验室条件下饲养4个月前后均达到鼠IgG含量低于5μg/ml的合格标准,5只SCID鼠腹腔移植入2或4×10~7个人外周血淋巴细胞(hu—PBL),建立人化SCID(hu-PBLSCID)鼠,可在移植后4周和12周测到其血清中人IgG的存在,最高可达109μg/ml。以脊髓灰质炎Ⅰ型病毒(Polio—1 virus,Polio-1)作抗原免疫5只hu-PBL-SCID各5次,每鼠均能产生抗Polio-1的特异性人抗体,滴度最高达1:640,表明所建立的人化SCID鼠具备了一定的免疫应答能力。更多还原

【Abstract】 Mouse IgG concentrations in serum obtained by tail vein bleeding,of all 10 SCID mice were found to be below the "non-leaky"level of 5ug/ml > before or after maintaining the mice in our laboratary for 4 months. In order to humanize the SCID mouse (hu-PBL-SCID) ,2 or 4X107 human peripheral blood lymphocytes were transplanted to 5 SCID mice. Four and twelve weeks later human IgG could be detected in their sera, the highest concentration being 109ug/ml. After repeatde (5 times) immunization of 5 hu-PBL-SCIDs with Polio-1, each of them could produce human anti-Polio-1 antibodies and the top litre amounted to 1 : 640. These results indicate that the hu-PBL-SCIDs does exhibit human immune response to some extents.更多还原