【摘要】 １９９０－１９９２年从新疆境内的多种婢、蚊、病人血清及脑脊液中分离到多株虫媒病毒，经初步鉴定，其中部分为甲属披膜病毒。１９９３年７～１０月与美国国家疾病控制中心（ＣＤＣ）虫媒传染病防治研究所合作，对上述病毒做进一步鉴定。３５株病毒感染Ｖｅｒｏ细胞，其中２８株可在３～７ｄ内引起典型的细胞病变。用２８株病毒进行空斑形成试验（ＰＦＴ），其中１９株可在Ｖｅｒｏ细胞中形成空斑，其滴度为ｌｏｇ１０４．７／ｍｌ～ｌｏｇ１０６．９／ｍｌ。对１９株病毒继续做血清空斑减数中和试验（ＳＤＰＲＮＴ），其中９１００６与屈曲病毒（Ｃｈｉｋｕｎｇｕｎｙａ，ＣＨＩＫ）和都光和病毒（ＤｏＱｕａｎｇＨａＴ５－７１，ＤＱＨＴ５－７１）抗体中和滴度为２５６０，库孜拉加奇病毒（Ｋｙｚｌａｇａｃｈ，ＫＹＺ）抗体中和滴度为１２８０、盖塔病毒（Ｇｅｔａｈ．ＧＥＴ）抗体中和滴度为３２０；９０１７９与鹭山病毒（Ｓａｇｉｙａｍａ，ＳＡＧ）抗体的中和滴度为１２８０，与ＣＨＩＫ、ＧＥＴ、ＫＹＺ和ＤＱＨＴ５－７１抗体中和滴度均为６４０，与辛德毕斯病毒（Ｓｉｎｄｂｉｓ，ＳＩＮ）抗体中和滴度为１６０；９１０１６０与ＣＨＩＫ抗体中和滴度为６４０，贝巴鲁病毒（Ｂｅｂａｒｕ，?更多还原

【Abstract】 In the period from 1990 to 1992, we isolated. early or late. some arboviruses from pools of ticks and mosquitoes as well as from the samples of sera and cerebrospinal fluid obtained from some patients; of those viruses some were preliminarily identified as the members of Alphavirus. However. some viruses could not be identified into a species due to the limited conditions in our country. In the period between July and October 1993. we worked in collaboralion with the scientists from the Institute of Vector-Borne infectious Disease of the Centers for Disease Control. U.S.A. in making a further identification of the above mentioned viruses. The results showed that of 35 strains of the viruses with which Vero cells were infected, 28 strains caused a typical cytopathic effect between 3 and 7 days, and other 7 ones did not so. A plaque formation test was conducted in the 28 strains which had caused a typical cytopathic effect in Vero cells. The results revealed that of those strains 19 had a plaque formation in Vero cells; the plague titres being log104.7/ml-log106.9/ml, and the 9 remainders did not so. A serum dilution-plaque reduction neutralization test was further conducted in the 19 strains which had gave a plaque formation in Vero cells. The results showed that 15 of the 19 strains, except 4 strains (No.91006. 90179. 910160 and 92081) which gave a cross neutralization reaction with the antibodies against some viruses of Alphavirus. gave no obvious cross neutralization reaction with the antibodies against some tested viruses (CHIK, GET, SAG, SF. SIN, KY, DQHT5-71, DQHT9-71 and BEB, etc), indicating that these viruses might be some new arbovirus species.更多还原