【摘要】 通过动物实验,利用组织学、组织化学和生物化学等手段,对同种透明软骨移植后不同时期基质中的蛋白多糖含量进行了动态变化观察。结果显示,蛋白多糖含量的高低对移植的异体软骨能否存活极为重要。更多还原

【Abstract】 The present work was to study the quantitative change of proteoglycan (PG)in homogenous cartilage graft in the guinea pig.Histological,histochemical and biochemical studies were undertaken at different times after grafting.The results re- vealed that there was a marked reducing trend of matrix PG within four weeks after grafting.Four weeks afterwards all indexes except hexuronic acid restored slowly and approached the level of the control group.By the 20th week,the activity of neutromu- copolysaccharide,hexosamine,acid-mucopolysaccharide and alkaline phosphatase re- stored to 73.6%～93.7% of the control group(P>0.05).The changes probably were caused by immune reaction and operative trauma. PG in the cartilage matrix forms a barrier for the chondrocytes,protecting the host’s immune system from contacting and reacting with the chondrocytes of the graft. Though the core protein of PG has antigenicity,it is surrounded by acidmu- copolysaccharide that has no antigenicity.We concluded that it is very important to maintain a high level of PG in order to prolong the survival of a cartilage graft.更多还原