【摘要】 用青石棉＋苯并（ａ）芘碘油混悬液对大鼠气管注入染尘，取染尘后１８０和２７０天剖检的肺组织标本２５例。提取标本中ＤＮＡ，用人工合成的寡核苷酸引物进行ＰＣＲ体外扩增，再用γ－３２ＰＡＴＰ标记正常和两种突变Ｋｉ－ｒａｓ１２位探针，杂交，放射自显影。结果表明，正常对照组７例标本Ｋｉ－ｒａｓ１２位点突变为阴性，青石棉＋苯并（ａ）芘组１８例标本中，３例Ｋｉ－ｒａｓ１２位点突变为阳性，其中２例由ＧＧＴ（Ｇｌｙ）突变为ＡＧＴ（Ｓｅｒ），１例由ＧＧＴ（Ｇｌｙ）突变为ＴＧＴ（Ｃｙｓ），并对突变原因进行了探讨。更多还原

【Abstract】 sample was prepared in iodized oil by mixing crocidolite(Cro.)to 20 mg per ml plus benzo(a)pyrene(B(a)P) to 50 mg perml.0.1 ml sample was injected intratracheally into each rat,once a month for 3 months. 25 lung tissue samples(7 normal control,18 Cro. plus B(a)P were obtained on the l80th and 270th day after the last injection. Ki-ras specific sequence of sample DNA wasamplified in vitro by PCR. The normal and two kind of mutation Ki-ras l2 codon probes were hybridized with salnple DNA. The re-sults showed:1)Ki-ras l2 codon mutation tests of 7 samples in normal control were negative;2)3 among the l8 samples in Cro. plus B(a)P group were positive in Ki-ras l2 codon mutation test. In regard to the pathological appearance of these 3 positive sam-ples,one was squamous cell carcinoma,the other two precancerosis or epitheliosis.更多还原