【摘要】 采用逆转录巢式ＰＣＲ技术检测了５１例ＨＣＣ患者的肝癌及癌周肝组织中ＨＣＶＲＮＡ正负链，同时以巢式ＰＣＲ技术检测了这些组织中的ＨＢＶＤＮＡ。结果，１１．８％（６／５１）检测出组织中ＨＣＶＲＮＡ正链；其中５例在癌周肝组织，２例在癌组织中检测出ＨＣＶＲＮＡ负链。由于负链ＲＮＡ为ＨＣＶ的复制中间体，不释放到细胞外，其检出为ＨＣＶ感染与ＨＣＣ关系的研究提供了进一步的病因证据。组织中ＨＢＶＤＮＡ检出率高达９２．２％（４７／５１），显示华南地区ＨＢＶ感染在ＨＣＣ的发生中起着特别重要的作用。更多还原

【Abstract】 Using reverse transcription(RT)nested polymerase chain reaction(PCR)technl que,the cancerous and pericancerous tissues from 51 patients with hep atocelluiar carcinoma(HCC)have been detected for plus and minus strands of HCV RNA and HBV DNA,6 patients specimens were positive of plus strands of HCV RNA, in which the minus strands of HCV RNA were found in 5 pericancerous specimens of liver tiSsue and 2 cancerous specimens.B ecause the minus stranded RNA is the replicative intermediate of HCV and can not be released to the outside of hepatocytes,its detectlon would Indicate the presence and replication of HCV inside hepatocytes.These results also demonstrated the relationship between HCV andHCC. The positlve rate of HBV DNA in HCC tissues detected by nested PCR was as hlgh as 92.2%,which indicated the iniportance of HBV in the development of HCC in Southern China.更多还原