【摘要】 从新生兔的四肢长骨中分离出破骨细胞，将其与大小约６ｍｍ×６ｍｍ、厚约１０μｍ的牛骨片共同培养。２４小时后，培养液中分别加入ＩＬ－２（５０Ｕ／ｍｌ，１００Ｕ／ｍｌ）；ＴＮＦ－α（１０￣（－１０）Ｍ，５×１０￣（－１０）Ｍ，１０￣（－９）Ｍ）；ＰＧＥ＿２（１００ｎｇ／ｍｌ）；并做空白对照。培养４０小时、６４小６寸及１周，利用相差显微镜计数吸收陷窝数，通过图像分析系统计算吸收陷窝表面积。结果表明：ＩＬ－２能促进破骨细胞性骨吸收：ＴＮＦ－α对分离破骨细胞性骨吸收无明显影响；ＰＧＥ＿２则对体外分离的破骨细胞具有抑制作用。更多还原

【Abstract】 AbstractOsteoclasts were mechanically isolated from one-day-old neonatal rabbit’s long bones and cultured onborine bone slices of approximately 6mm × 6mm in sizeand 1μm in thickness. after 24 hours,each bone slicewas placed in a well of 24-well culture plate containingIL-2（50u/ml,100u/ml）, TNF-α（10￣（-10） M,5× 10￣（-10）M,10（-9）M）,PGE₂（100ng/ml ） in medium 199,orcontrol. When they had been cuItured for 40 hours,64 hours and 1 week, the number of resorptionlacunae on each slice was counted under phase-contrastminroscope.In the same time,pictures were takenand used to count surface area of lacunaeby In thesLame time pictures were taken and used to countsurface area of lacunae by Interreactive lmage Analysissvstem The initial results show:L-2 stimulatedosteoclastic bOne resorption; TNF-α has notsignificant effect on osteoclastic bone resorption; whilePGE₂ inhibited osteoclastic bone resorption.更多还原