【摘要】 利用ＰＣＲ技术和免疫组化方法，对２８例滤泡型淋巴瘤和１８例反应性滤泡增生进行了ｂｃｌ－２／ＩｇＨ融合基因和ｂｃｌ－２蛋白表达的检测。结果发现不仅５７％的滤泡型淋巴瘤中ｔ（１４：１８）易位，而且在３３％的滤泡反应性增生也存在这种易位。二者经Ｘ￣２检验无显著性差异（Ｐ＞０．０５）。在本实验中仅采用ｍｂｒ引物对的条件下，ｂｃｌ－２蛋白异常表达与ｂｃｌ－２／ＩｇＨ融合基因的形成之间并无相互对应的关系。在肿瘤性滤泡与反应性滤泡中ｂｃｌ－２蛋白表达的不同分布，有助于对两者的鉴别诊断。更多还原

【Abstract】 olymerase chain reaction(PCR) and immunohistochemical staining methods were used to detect the bcl-2/IgH fusion gene[t(14:18)]and the overexpression of bcl-2 protein in 28 cases of follicular lymphornas and 18 cases of reactive follicular hyperplasia. The results showed that the bcl-2/IgH fusion gene was present in 57/of follicular lymphomas and that this translocation was also present in 33% of the reactive follicular hyperplasia cases. The over expression of bcl-2 protein was found in 78%of follic-ular lymphomas, but none in follicular hyperplasia,therefore bcl-2 protein staining may be a useful im-munohistochemical method to differentiate follicular lymphoma and follicular hyperplasia.更多还原