【摘要】 用Ｎｏｒｔｈｅｒｎ核酸杂交技术对４个人肺癌细胞系进行了表皮生长因子（ＥＧＦ）、转化生长因子α（ＴＧＦα）以及表皮生长因子受体（ＥＧＦＲ）的基因表达研究，发现这些肺癌细胞系中均有ＥＧＦ和ＴＧＦ＿α的基因表达，３／４中亦有ＥＧＦＲ的基因表达。这些癌细胞系中自泌的生长因子作用于自身的ＥＧＦ受体形成的自泌循环，不断刺激其自身的增殖，这可能是癌细胞无休止生长的主要原因之一。Ｎｏｒｔｈｅｒｎ核酸杂交结果还表明：在这４个肺癌细胞系中，无ｃ－ｍｙｃ癌基因表达的细胞系中有大量的ＥＧＦ和ＴＧＦα的基因表达。用ＥＧＬ抗ＥＧＦ和抗ＥＧＦＲ抗体处理这些人肺癌细胞系，抗ＥＧＦ和抗ＥＧＦＲ抗体在一定程度上可抑制其增殖，这也说明这些癌细胞中生长因子自泌环路的存在。更多还原

【Abstract】 ene expression of growth factors including epidermal growth factor (EGF),transforming growth factor α(TGFα),epidermal growth factor receptor(EGFR),oncogenes such as c-myc,N-ras,c-erbB2 and tumor suppressor gene P53 were studied in 4 human lung cancer cell lines using Northern blot tech-nique. Among these cell lines were 2 adenocarcinoma cell lines, one large cell carcinoma cell line and one small cell carcinoma cell line. Expression of EGF and TGFα mRNAs were found in all 4 cell lines and E-FGR mRNA was seen in 3 out of 4 cell lines. Among these cell lines, 2 cell lines with weaker expression of EGF and TGFα,expressed c-myc mRNA. Another 2 cell lines had no c-myc but expressed large amounts of EGF and TGFα mRNA. No expression of N-ras,c-erbB2 and p53 were found in these cell lines. The results indicate the presence of autocrine loop of growth factors in these cancer cells. The au-tostimulation of growth factors may be the main cause for the uncontrolled growth of cancer cells. After treating the cancer cells with EGF anti-EGF and anti-EGFR antibodies, EGF was found to exert a mild stimulating effect on the growth of one cell line, but no effect on the other cell lines. Anti-EGF and anti-EGFR antibodies inhibited the cell growth on all cell lines. These results provided further evidence for the presence of autocrine loop of growth factors in these lung cancer cell lines.更多还原