【摘要】 应用⁵¹Cr标记人的多发性骨髓瘤（MM）细胞系U266、XG-7,着重研究了细胞因子对人MM系U266、XG-7——内皮细胞间粘附的调控作用及其对细胞表面粘附分子表达、细胞因子分泌的影响。主要结果表明:（1）IL-6及其IL-6Rgp130相关性生长因子（GM-CSF等）不仅是人MM细胞体内、外的生长因子,而且可以提高MM细胞——内皮细胞间的粘附能力,有助于MM的扩散。（2）细胞因子不仅可以诱导内皮细胞表面CD54、CD44表达的增加,而且可以诱导其分泌IL-6、TNF等可溶性因子;粘附亦能引起肿瘤细胞XG-7表面CD11a,CD54,CD44,VLA-4的异常改变;同时MM细胞——基质细胞的相互作用可以诱导MM细胞迅速自分泌IL-6。（3）由细胞因子和粘附分子调控的“基质细胞——肿瘤细胞”相互作用是人MM发生发展的重要环节。其中,VLA-4及其配体的相互作用可能参与了MM细胞与内皮细胞的粘附过程。更多还原

【Abstract】 Using 51Cr labelled multiple myeloma (MM) cell lines U266/XG-7, the regulatory effect of cytokines on the adhesive interaction between myeloma-cell lines U266/XG-7 and the endothelial cells, and the effects of these cytokines on expression of adhesion molecules and secretion of other cytokines were studied. The experimental results were as follows- (1) IL-6 and IL-6Rgpl30-associated growth factors (such as GM-CSF) are not only myeloma cell growth factors, but also can enhanced the adhesion between MM cells and endothelial cells and thus facilitated the metastasis of tumor cells. (2) Cytokines could induce increase in the expression of CD54 and CD44 on the endothelial cells and the secretion of IL-6 and TNF by the endothelial cells. On the other hand, the adhesion could also cause the change of CDlla, CD54, CD44 and VLA-4 on surface of myeloma cells XG-7. Finally, the interaction between MM cells and stromal cells from murine bone marrow could rapidly induce autocrine of IL-6 in human IL-6-dependent MM cells. (3) The interaction between stromal cells and tumor cells regulated by the cytokines and adhesion molecules was a key element in the pathogenesis and development of human MM. Among these factors, VLA-4 might be one of the molecules involved in U266/XG-7-EC interaction.更多还原