【摘要】 在ＨＬ－６０白血病细胞的体外培养中，组胺Ｈ＿２受体激动剂４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有轻微的抑制作用，而组胺Ｈ＿２受体拮抗剂雷尼替丁（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０细胞的增殖具有较强的抑制作用。但用１０￣（－６）ｍｏｌ／Ｌ的雷尼替丁预处理ＨＬ－６０细胞则可部分地拮抗４－甲基组胺（１０￣（－８）－１０￣（－４）ｍｏｌ／Ｌ）对ＨＬ－６０白血病细胞的抑制作用。更多还原

【Abstract】 The results obtained from liquid suspension culture,semisolid culture and ￣3H-TdR incorpo-ration into HL-60 leukemia cells indicated that histamine H₂ receptor agonist 4-methylhistamine（4-MH） had slight suppressive effect on HL-60 leukemia cells when the 4-MHdoses were 10￣（-8）-10￣（-4）mol / L.The IC₅0 of 4-MH in HL-60 colony culture was 1.74 ×10￣（-5）mol / L. Histamine H₂ receptor antagonist ranitidine exerted its suppressive effect onHL-60 leukemia cells when ranitidine 10￣（-8）mol / L to 10￣（-4）mol / L were added.The IC₅0 ofranitidine in HL-60 colony culture was 4.24×10￣（-8）mol / L.Therefore, histamine H₂ receptor an-tagonist ranitidine exerted greater suppressive effect on HL-60 leukemia cells than histamine H₂receptor agonist 4-MH did.When HL-60 leukemia cells were pretreated with ranitidine10￣（-6）mol / L for 0.5h,which had no supressive effect on HL-60 cells,the inhibitory effect of4-MH（10￣（-8）-10￣（-4）mol / L） on HL-60 leukemia cells could be antagonized to some extent.Thesephenomena suggest that histamine H₂ receptor agonist 4-MH and its antagonist ranitidine mayexert their suppressive effects on HL-60 leukemia cells through different mechanisms whichshould be clarified in further experiments.更多还原