【摘要】 本实验采用同位素〔￣３Ｈ〕－肌醇参入与阴离子交换树脂层析分离的方法，比较了Ｗｉｓｔａｒ大鼠与两肾一环肾性高血压大鼠（２Ｋ－ＩＣＲＨＲ）主动脉与尾动脉磷酸肌醇（包括－磷酸肌醇ＩＰ、二磷酸肌醇ＩＰ＿２和三磷酸肌醇ＩＰ＿３）的含量，以及α＿１－肾上腺素能受体（α＿１－受体）激动剂苯肾上腺素（ＰＥ）对它们的影响。结果发现：（１）无论是在静息还是在ＰＥ激活的状态下，血管平滑肌中的磷酸肌醇均以ＩＰ为主，约占总磷酸肌醇的７１－８２％；（２）尾动脉中ＩＰ＿２与ＩＰ＿３的基础含量明显高于主动脉，提示尾动脉中磷脂酶Ｃ（ＰＬＣ）的基础活性高于主动脉；（３）在ＰＥ（１０￣（－５）ｍｏｌ／Ｌ）的作用下，各标本中ＩＰ含量均明显增高，反映了ＰＬＣ被激活。ＩＰ增加的幅度在尾动脉高于主动脉，而以ＲＨＲ尾动脉为最大，但各标本中ＩＰ＿２与ＩＰ＿３含量未见明显变化。ＰＥ作用后，ＲＨＲ尾动脉中ＩＰ含量明显高于Ｗｉｓｔａｒ大鼠。这表明尾动脉α＿１受体－ＰＬＣ通路的活性高于主动脉；（４）基础状态下，尾动脉ＩＰ在总磷酸肌醇中所占比例低于主动脉，ＰＥ激活后ＩＰ所占比例明显增高，而主动脉上述比例无明显变化。提示主动脉与尾动脉被ＰＥ激活后，磷酸肌醇代谢途径和方?更多还原

【Abstract】 By means of ￣3H myo-inositol labelling and ion exchange chromatography, contents ofinositol phosphates, including IP, IP₂ and IP₃, were studied in both aorta and tail artery ofWistar rats and renal hypertensive rats（RHR, two kidney, one clip）.Effects of phenylephrine（PE）,an α₁ agonist,on such contents were also observed. The results were as follows:（1）In bothresting and PE-activated arteries, IP took the greatest part（71─82%）in total amount ofinositol phosphates.（2）The basal contents of IP₂ and IP₃ were significantly higher in tail arterythan in aorta, suggesting that tail artery has higher basal activity of phospholipase C than aorta.（3）PE（10￣（-5）mol/L）treatment significantly increased IP contents in all of the arteries, reflectingthe activation of PLC by PE. while no significant change was observed in contents of IP₂ andIP₃ after PE activation. The increment of IP content was more significant in tail artery than inaorta, with the largest elevation found in tail artery of RHR. These indicate that the activity ofα₁ receptor-PLC pathway is higher in tail artery than in aorta.（4）Under basal condition,theproportion of IP in total inositol phosphates was lower in tail artery as compared with aorta. PEactivation could increase such proportion in tail artery but not in aorta, suggesting that the reac-tion pattern of PLC and phosphatase is different between the tail artery and aorta.更多还原