【摘要】 目的:观察氟哌利多与电针(EA)合用后前脑啡肽原(PPE)mRNA的表达.方法:氟哌利多与电针合用后取大鼠脑切片,以非放射性的原位杂交组织化学技术观察PPE mRNA.结果:电针10 h之后,PPE mRNA的表达增强了;而EA与氟哌利多合用后,PPE mRNA的表达在许多与痛觉调制有关的核团被进一步增强.如尾壳核,伏核,隔核,斜角带核,杏仁核,下丘脑,中脑导水管周围灰质,脚间核,黑质,脊髓背角.结论:氟哌利多促进电针对PPE mRNA表达的作用,与其加强针刺镇痛的机制有关.更多还原

【Abstract】 AIM:To study the expression of pre proenkephalin (PPE) mRNA following elec-troacupuncture (EA) combined with droperi-dol (Dro), an antagonist of dopamine receptors. METHOD:The brains of Sprague-Dawley rats were sectioned after combination of EA with Dro and nonradioactive in situ hybridization histochemistry (ISHH) technic was used. RESULTS: Ten hours after EA, the expression of PPE mRNA was enhanced; when EA was combined with Dro, the expression of PPE mRNA was further enhanced in many pain-modulation-related nuclei, such as caudate-putamen, accumbens, septal nucleus, diagonal band nucleus, amygdala, hypothala-mus, periaqueductal gray (PAG), interpedun-cular nucleus, substantia nigra, and the dorsal horn of spinal cord (layer Ⅰ-Ⅱ and Ⅲ-Ⅳ). CONCLUSION: Dro promoted EA’s action on the PPE mRNA expression, which underlie the mechanisms of Dro potentiation on acupuncture analgesia.更多还原