【摘要】 采用HPLC法测定了银杏叶浸膏干粉及银杏叶胶囊中总黄酮含量。采用YWG－C18柱，甲醇－水－冰醋酸（40：57．5：2．5，V／v）为流动相，检测波长254nm。样品中10种成分分离较好以芦丁为对照外标法定量，线控范围0．5～2．5μg，r＝0．9996，加样回收率为104．2％（RSD＝3．3％）。更多还原

【Abstract】 Quantitative HPLC method was developed for the determination of flavonoids in extract preparations of leaves of Ginkgo biloba L. YWG-C13 column was used with a mobile phase of methanol-water-glacial acetic acid (40: 57.5 : 2.5, V/V ) and detected at 254nm.The flow rate was 1.0ml/min. Ten Peaks were observed. Rutin was used as external standard and the calibratiott cirve was linear over the rang of 0.5~2.5μg (r =0.9996 ). The extraction recovery was 104.2% (RSD = 3.3% ) .Compared with the res ilts of chemical colorimetric analysis, the method has a better reprod icibility and more information about the flavonoids in G. biloba can be obtained.更多还原