【摘要】 为探讨血小板活化因子（ＰＡＦ）对肾小球系膜细胞（ＧＭＣ）产生活性氧的调节作用，本研究观察了ＰＡＦ对体外培养的大鼠ＧＭＣ产生超氧化物阴离子（Ｏ）和过氧化氢（Ｈ－２Ｏ－２）的影响。结果表明１０￣（－９）Ｍ／Ｌ的ＰＡＦ已能诱导ＧＭＣ产生Ｏ和Ｈ２Ｏ２（１．１４±０．４２ｎｍｏｌ／１０￣５ＧＭＣ、０．９７±０．１６ｎｍｏｌ／１０－５ＧＭＣ），这一效应呈剂量依赖性；溶ＰＡＦ无诱导ＧＭＣ产生活性氧作用；特异性ＰＡＦ受体拮抗剂ＢＮ５２０２１抑制ＰＡＦ的刺激作用，抑制作用也呈剂量依赖性，５×１０￣（－５）Ｍ／ＬＢＮ５２０２１完全抑制ＧＭＣ合成活性氧。本研究说明ＰＡＦ可诱导ＧＭＣ合成活性氧。更多还原

【Abstract】 In order to investigate the regulatory effect of platelet activating factor(PAF)on the production of reactive oxygen species(ROS) from glomerular mesangialcells(GMC),the effect of PAF on the production of superoxide anion(O)and hydro-gen peroxide( H-2O-2 ) by the cultured rat GMC in vitro was studied. Data showed that10￣(9) mol/L of PAF led GMC to produce O and H-2O-2(1.14 ± 0.41 nmol/10￣5 GMC,0.97 ± 0.16 nmol/10￣5 GMC).This response was dose-dependence. Lyso-PAF did notevoke GMC to produce ROS. Specific PAF receptor antagonist,BN 52021, inhibited theeffect of PAF on GMC,this response was also dose-dependence,and the level of 5 ×10￣(-5)mol/L BN 52021 completely suppressedthe production of ROS by GMC.Theseresults suggested that PAF may induce production of ROS by OMC, and that PAF anta-gonist might be a good medicine for prevention and treatment of kidney diseases.更多还原