【摘要】 采用１２５Ｉ－ＵｄＲ同位素释放试验，观察利巴韦林（三氮唑核苷，ＲＴＣ）在体外对小鼠脾细胞自然杀伤（Ｎｋ）活性的影响。结果表明，ＲＴＣ的剂量与ＮＫ活性呈负相关，在５ｕｍｏｌ·Ｌ－１时ＲＴＣ即可显著抑制ＮＫ活性。ＲＴＣ与地塞米松合用对ＮＫ活性的抑制有相加作用，而ＲＴＣ与γ干扰素合用则互为拮抗作用。此外，还发现ＲＴＣ能明显降低刀豆蛋白Ａ诱生的小鼠脾细胞干扰素及白细胞介素－２的产量，推测ＲＴＣ对ＮＫ活性抑制的部分机理是阻碍了这些淋巴因子的生成。更多还原

【Abstract】 The effects of ribvirin(1-β-D-ribofuranosy 1-1,2,4-triazole-3-carboxamide,RTC)on natural killer(NK)activities of murine spleen cells in vitro were studied by125I-UdR isotoperelease assay.The results showed that RTC at the concentration of 5 umol·L-1 significantlysuppressed NK activities(P<0.01),and a negative correlation between NK activities and RTCdosage was obtained.It also indicated that there was additive effect between the actions of RTC anddexamethasone(Dex,1 umol·L-1),and antagonism between the actions of RTC and interferon-γ(100 U·ml-1).In addition,RTC at the concentration of 0.5 umol·L-1 was found to be enough toreduce the product of interferon(P<0.01)and interieukin-2(P<0.05)induced by Con A in murinespleen cells,and a negative dcoe-effect relationship was obeerved.Thus,one of the mechanisms ofRTC in inhibiting NK activities seems to suppress the production of these lymphokines.更多还原