【摘要】 以美托洛尔为内标，建立了ＨＰＬＣ－ＵＶ测定大鼠血浆、尿、粪、胆汁及肝脑组织匀浆中药物浓度的方法。流动相为甲醇、乙腈、水及０．２ｍｏｌ·Ｌ－１醋酸钠缓冲液，以Ｃ１８柱分离，紫外检测波长２３２ｎｍ，生物样品在不同条件下经两次乙醚处理可获得良好分离。血浆样品最低检测浓度为５ｎｇ·ｍｌ－１，日内ＲＳＤ和日间ＲＳＤ为３．１７～１０．８８％，平均回收率为７９．３５～９５．７２％。本法简便、灵敏、可靠，经大鼠单次灌胃２０ｍｇ·ｋｇ－１后血药浓度的测定验证，可满足临床前药代动力学研究的需要。更多还原

【Abstract】 A method was developed for the determination of the novel antihypertensive drugnaftopidil in biological samples by HPLC,Metoprolol was used as the internal standard.The analyticalcolumn was a stainless steel column filled with 10 μmC1s packing.The mobile phase was a mixture ofmethanol-acetonitrile-water-0.2 mol·L-1Ac-0.2 mol·L-1aAc(50 :45 :5:0.9:0.1).Detection was performed at UV 232 nm,Biologieal samples can be well purified after twoextractions with ether.The lowest detection limit was 5 ng· ml-1.The precision and accuracywithin-day and day-to-day ranged from 3.17 to l0. 88%,The mean recoveries were 79.35%to95.72%,The results showed that this method was simple,sensitive and good enough to be used inpharmacokinetic study of naftopidil。更多还原