【摘要】 戊二醛交联的酵母细胞，包埋于聚乙烯醇中，制成含有糖酵解部分酶系的固定化多酶体系颗粒。该固定化多酶体系可以以葡萄糖为底物，通过糖酵解途径生产１，６－二磷酸果糖（ＦＤＰ）。为了使ＦＤＰ大量积累，选择氯丙嗪作为醛缩酶抑制剂，再偶联上ＡＴＰ再生系统，连续批式生产ＦＤＰ。当再生的ＡＴＰ浓度达０．２ｍｇ／ｍｌ时，ＦＤＰ产量可提高数倍。批式反应，连续生产５批，ＦＤＰ产量可维持在１８．５ｍｇ／ｍｌ。结果表明，采用再生的方法来补充ＡＴＰ，可保证固定化酵母细胞连续生产ＦＤＰ。更多还原

【Abstract】 n Immobilized multiple enzyme particles,which were prepared by enhancing glutaraldehyde crosslinked yeast cells in polyvinyl alcohol(PVA),could catalyze the reactions to preduce fructose-1,6-diphosphate(FDP) from glucose. In order to accumulate FDP, chlorpromazine was selected as an in-hibitor to block the further reactions and a coupled ATP regeneration system was used to supply ATPfor the reactions.更多还原