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枫泾猪、香猪和长白猪的DNA指纹图分析

Analysis of DNA Fingerprints of Fengjing, Xiang and Large White Pigs

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【作者】 孟安明; 齐顺章; 于汝梁; 王瑞祥; 蔡正华; 焦淑贤; 辛彩云; 张志武;

【Author】 Meng Anming; Qi Shunzhang (College of Biology, Beijing Agricultural Univcrsity, 100094)Yu Ruliang; Wang Ruixiang; Cai Zhenghua ;Jiao Shuxian; Xin Caiyun; Zhang Zhiwu(Institute of Domestic Animals Science, Chinese Academy of Agricultural Sciences, Beijing

【机构】 北京农业大学生物学院!100094; 中国农业科学院畜牧研究所!北京; 100094; 中国农业科学院;

【摘要】 本文用M13和3个人源小卫星探针(33.6、33.15、α-珠蛋白-3’HVR)获得了枫泾猪、香猪及长白猪的DNA指纹图,分析了各品种内和品种间的遗传变异性.结果表明,各品种的平均DNA指纹图相似系数在0.440-0.532的范围内,极显著地高于品种间的相似系数(0.223-0.307)。各品种的DNA指纹图还存在着一些品种特异性图带,这些图带在以后的基因定位中值得重视.

【Abstract】 This paper demonstrated DNA fingerprints of Fengjing, Xiang and Large White breeds of swine generated by M 13 and three human minisatcllite probes (33.6, 33.15 and α-globin-3’HVR), and determined the intrabreed and interbreed variabilities of swine DNA fingerprints. It was shown that the mean band sharing probability within breeds was 0.440-0.532, significantly higher than that between breeds (0.223-0.307). Some breed-specific bands were noted in each breed, which should draw our attention in the course of gene mapping.

  • 【分类号】Q953
  • 【被引频次】28
  • 【下载频次】66
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