【摘要】 本文报道了一种检测二核苷酸重复多态性的简便的非同位素法，利用重复序列两侧的特异引物进行ＰＣＲ扩增，产生的等位片段在薄层变性聚丙烯酰胺凝胶电泳上分离，再用灵敏的银染法显色。该方法不需要标记ＰＣＲ产物，简便、快速，分辨率可达１ｂｐ，并可用多对引物同时进行多重ＰＣＲ分析。用此方法对ＤＭＤ家系成员ｄｙｓｔｒｏｐｈｉｎ基因的５＇－脑型外显子上游区和３＇－非翻译区的两个（ＣＡ）ｎ位点进行了扩增片段长度多态性分析，表明它是连锁分析和基因诊断的一种方便有效的新技术。更多还原

【Abstract】 We have developed a rapid and non-radioactive procedure for detecting dinucleotide repeat polymorphisms.Specific primers flanking the repeats were used in thepolymerase chain reaction(PCR).The generated allelic fragments were resolved bythin-layer denaturing polyacrylamide gel electrophoresis and visualized by improvedsilver staining. This method is simple, fast and without labelling of PCR products.Resolution of 1 bp can be easily obtained. It can also be used in multiplex PCR analysis of(CA)n loci. This method will be a valuable tool for linkage analysis andDNA diagnosis as demonstrated by its successful application to the CA dinucieotiderepeat haplotying of a DMD family.Received October 29.1993更多还原