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DNA-蛋白交联物在人群中的应用及其正常值初探

Ren Zefaug , et al. Application of DNA-proteincross links in human and estimation of it normal va-lue

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【作者】 任泽舫庄志雄刘移民邓丽霞刘力张桥

【Author】 .

【机构】 中山医科大学卫生学院

【摘要】 利用简便、快速、经济、敏感的 ̄(125)I后标记法检测某水电站47名工人的血白细胞DNA-蛋白交联物(DPC),结果显示性别、年龄、文化程度、吸烟、饮酒等对DPC的量无影响。该人群的DPC(cpm/μg)呈正态分布,均数为1446.4,标准差为396.5。本文还介绍了 ̄(125)I后标记法检测DPC的原理和方法。

【Abstract】 DNA-protein cross links in the peripheral whiteblood cells of 47 workers in a hydropower stationwere detected with ̄125I postlabelling assay, a simple,rapid , inexpensive, and sensitive method.The resultshowed that no marked difference of DPC formationbetween sex ,age,culture level,smoking and alcoholdrinking was found.The frequeneies of DPC in thegroup was in normal distribution.The mean and stan-dard deviation was respectively 1446.4 and 396.5(cpm/μg DNA).The principle and method of  ̄125Ipostlabelling assay was introduced.

  • 【分类号】R135.1
  • 【被引频次】2
  • 【下载频次】41
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