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L929细胞株生物学特性的初步研究
BIOLOGICAL CHARACTERISTICS OF L929 CELL LINE
【摘要】 对L929细胞株的生物学特征进行了初步研究。结果表明,该L929细胞株是一株易于培养、集落清晰的雄性细胞。集落形态分为致密型与疏散型2种,集落生成数与血清浓度在1%~10%之间呈明显剂量依赖性,细胞倍增时间25.49小时。L929细胞株的生物学特征符合成纤维细胞的生物学特征。
【Abstract】 The biological characteristics of L929 cell line were investigated. It was found that L929 cell line was a male cell line which was easy to culture and formed clear colonies. Colonies could be divided into two types: dense type and spersed type. It showed positive correlation be-tween the number of colony formation and 1%-10% serum concentrations. The doubling time of L929 cell was 25.49 hrs. It is in accordance with the biological characteristics of fibroblasts. This investigation provided us a good modei for the study of cells.
【关键词】 L929细胞株;
L929-CM;
细胞培养;
染色体;
融合状态;
【Key words】 L929 cell line L929- cell conditioned medium cell culture chromosome conflueut layer;
【Key words】 L929 cell line L929- cell conditioned medium cell culture chromosome conflueut layer;
【基金】 国家自然科学基金
- 【文献出处】 实验血液学杂志 ,Journal of Experimental Hematology , 编辑部邮箱 ,1995年04期
- 【分类号】R329
- 【被引频次】9
- 【下载频次】460