【摘要】 应用斑点免疫金银染色法(Dot-IGSS)和Dot-ELISA检测96份确诊为脑囊虫病患者脑脊液(CSF)中的循环抗原(CAg),阳性率分别为94.79%和92.71%.检测51例非囊虫病者和62例其他脑部疾病患者的CSF,两种方法均为阴性.诊断指数分别为194.79%和192.71%,检测CAg的最小量Dot-IGSS为244ng/ml,Dot-ELISA为488ng/ml.试验表明两法诊断脑囊虫病均具有敏感性高、特异性强、重复性好和方法简便易行,有希望用于脑囊虫病的临床诊断.更多还原

【Abstract】 Detection of circulating antigens (CAg) of cerebrospinal fluid (CSF) by Dot-im-munogold-silver staining (Dot-IGSS) and Dot-ELISA were performed for diagnosis of 96 confirmed cases of cerebral cysticercosis. The positive rate of the Dot-IGSS and Dot-ELISA were 94.79% and 92.71% respectively.Detection of CSF from 51 patients with non-cysticercosis and 62 patients with other cerebral diseases were all negative by these two methods. The detection indices was 194.79% and 192.71% respectively. The minimal amount of CAg which could be detected was 244ng/ ml by Dot-IGSS and 488ng/ml by Dot-ELISA.The results indicated that both methods were highly specific,sensitive, reproducible and promising for the diagnosis of cerebral cysticercosis.更多还原