【摘要】 本文用流式细胞仪测定了NaNO₂对体外培养的Wistar乳鼠心肌细胞周期的影响。结果表明,10^-6mol/L NaNO₂引起S期细胞明显减少（P<0.05）;10^-6mol/L NaNO₂对其作用不显著（P>0.05）。应用[³H]TdR掺入法测定了NaNO₂对心肌细胞增殖的作用。实验发现,10^-6mol/L NaNO₂明显抑制细胞增殖,而10^-9-10^-7mol/L的NaNO₂则促进细胞增殖,以10^-8mol/L的NaNO₂作用最强。NaNO₂还能引起心肌细胞过氧化脂质含量增加。更多还原

【Abstract】 In our previous work we have demonstrated that sodium nitrite caused acute myocardial necrosis in rats in vivo. The direct effect of sodium nitrite on cell cycle in cultured neonatal rat heart cells and its mechanism were investigated with the following results-. (1) large doses of sodium nitrite (10-6mol/L) could decrease the percentage of S phase of myocytes (P<0.05) (2) The effects of sodium nitrite on [3H]TdR incorporation into DNA of myocytes were; small doses (10-9- 10-7mol/L) was stimulatory, while large dose (10-6mol/L) was inhibitive. (3) Sodium nitrite (10-8-10-6mol/L) could increase the melondialdehyde contents of myocytes. These results suggested that the changes of myocytes induced by sodium nitrite were relative to lipid peroxidation.更多还原