【摘要】 采用限制酶酶切片段长度多态性（RFLP）分析方法比较了水稻野败不育系及其籼型保持系线粒体DNA酶切图谱。野败不育系与籼型保持系线粒体DNA在组成上存在十分明显的差异。根据具有相同迁移位置与大小的片段比例判断,这2种不同细胞质线粒体DNA顺序同源性约为30％。分子杂交结果显示,编码功能蛋白的基因及其邻近顺序存在相当程度的保守性。本文并对线粒体DNA的组成变异与细胞质雄性不育的关系进行了分析与讨论。更多还原

【Abstract】 Restriction patterns of mitochondrial DNA were compared between male sterile line of wild abortion and maintainer line of Indica rice via RFLP analysis. There were distinct differences of mtDNA compositions between these two lines. Judged from the ratio of those fragments in the same migration rates, there was 30% homologous sequences between these two lines. Southern hybridization results showed that coding sequences and neighboring sequences of genes were relatively conserved. Discussion was made on the relationship between the change of the DNA composition and the male sterile cytoplasm.更多还原