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QTL区间作图的统计理论和计算机软件及其应用

A Statistical Technique and Generalized Computer Software for Interval Mapping of Quantitative Trait Loci and Its Application

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【作者】 徐云碧申宗坦陈英朱立煌

【Author】 Xu Yunbi Shen Zongtan(Agronomy Department, Zhejiang Agricultural University, Hangzhou 310029)Chen Ying Zhu Lihuang(Institute of Genetics, Chinese Academy of Science, Beijing 100101)

【机构】 浙江农业大学农学系!杭州310029中国科学院遗传研究所!北京10010l

【摘要】 本文系统地描述了应用两个相邻共显性分子标记进行数量性状基因座位(QTL)区间作图的方法。编写了可在容量较小的微型机上运行,适合于F2、DH,BC等群体QTL作图的通用计算机程序QTLMAP-I。并给出了将这一方法应用于水稻广亲和基因及与抽穗期和每穗粒数有关的QTL区间作图的实例,将广亲和基因S5n定位于色素原基因C和RG138之间68cM处,证实了采用质量基因定位方法所得的结果。

【Abstract】 A statistical method was described for interval mapping of quantitative trait loci (QTL) using codominant flanking molecular markers such as RFLPs. A generalized computer software, QTLMAP-Ⅰ, suitable for use on a personal computer with low memory capacity was designed for different mapping populations including F2、BC and DH. As an example, the method was used to map QTLs related with wide compatibility, days to heading and grain number per panicle in rice. The gene for wide compatibility, S5n, was located on the interval between the chromogen locus (C) and an RFLP marker (RG138), and its distance from C was 3 cM, which confirmed the results obtained by use of the method for qualitative characters.

【基金】 国家高技术计划生物技术领域青年科学基金资助项目
  • 【文献出处】 作物学报 ,Acta Agronomica Sinica , 编辑部邮箱 ,1995年01期
  • 【分类号】S114
  • 【被引频次】38
  • 【下载频次】585
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