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氯化钯—分光光度法测定油菜籽硫苷含量的研究
Determination of Glucosinolate Content in Rapeseed with Palladium Chloride Spectrometry Method
【摘要】 利用生物统计方法对用于低硫苷油菜育种后代材料筛选、定量的的氯化钯—分光光度法操作的可靠性及适用条件进行了分析,结果表明:①采用1次重复测定可对硫苷含量差值为0.048mg/g的种子样品作出正确的分辨,用于育种后代材料筛选;②采用2次重复测定的平均值可对油菜—类种子的硫苷含量给出较准确的估计。
【Abstract】 The reliability of palladium chloride-spectrometry method and its appli-cation condition in material screening with low glucosinolate in rapeseed breeding havebeen discussed with statistical method in this paper.The results show that:1.determina-tion with one repeatation can produce a correct differentiation for total glucosinolatecontent of Brassicas oil seeds with different values of 0.048 mg/g for the materialscreening in early generation;2.average value determined with two repeatations can re-sult in an accurate estimate for Brassicas oil seed.
【关键词】 油菜;
硫苷分析;
氯化钯—分光光度法;
【Key words】 repeseed; glucosinolate analysis; palladitim chloride spectrometry;
【Key words】 repeseed; glucosinolate analysis; palladitim chloride spectrometry;
【基金】 陕西省“八五”攻关项目
- 【文献出处】 西北农业大学学报 ,ACTA UNIVERSITATIS AGRICULTURAE BOREALI-OCCIDENTALIS , 编辑部邮箱 ,1995年06期
- 【分类号】S565.401
- 【被引频次】7
- 【下载频次】478