【摘要】 用５％二甲基亚砜＋１０％葡萄糖或５％二甲基亚砜＋１０％蔗糖作为冰冻保护剂，能有效减轻中华猕猴桃愈伤组织细胞的超低温伤害。以－１℃／ｍｉｎ的速率降温至－６０～－７５℃时，对愈伤组织细胞的伤害作用最小；以大于１６０℃／ｍｉｎ速率快速化冻，愈伤组织细胞的相对存活率最高。愈伤组织细胞伤害的主要温度区域为－２０～－４０℃；－６０～－７５℃为愈伤组织细胞投入液氮时的“安全温度区”，－６０℃和－７５℃分别为“临界上限温度”和“临界下限温度”。更多还原

【Abstract】 Being as cryoprotectants,5%DMSO- 10%glucuse or 5%DMSO- 10%sucrose could greatly reduce the injury of Actinidia chinensis callus cells. The in-jury of callus cells was minimal when freezed at the rate of- 1℃/min.to-60～-70℃。 The survival rate of the callus cells was maximal when rewarmed at the rate ofmore than 160℃/min. -20～-40℃ was the major temperature scope of the callus cellinjury;-60～- 75℃was the security temperature scope when callus cells were im-mersed in liquid nitrogen;-60℃ and-75℃ were the upper and lower limits of criticaltemperatures respectively.更多还原