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中华猕猴桃愈伤组织细胞的超低温伤害研究
The Injury of Actinidia chinensis Callus Cells inSuper-low Temperature
【摘要】 用5%二甲基亚砜+10%葡萄糖或5%二甲基亚砜+10%蔗糖作为冰冻保护剂,能有效减轻中华猕猴桃愈伤组织细胞的超低温伤害。以-1℃/min的速率降温至-60~-75℃时,对愈伤组织细胞的伤害作用最小;以大于160℃/min速率快速化冻,愈伤组织细胞的相对存活率最高。愈伤组织细胞伤害的主要温度区域为-20~-40℃;-60~-75℃为愈伤组织细胞投入液氮时的“安全温度区”,-60℃和-75℃分别为“临界上限温度”和“临界下限温度”。
【Abstract】 Being as cryoprotectants,5%DMSO- 10%glucuse or 5%DMSO- 10%sucrose could greatly reduce the injury of Actinidia chinensis callus cells. The in-jury of callus cells was minimal when freezed at the rate of- 1℃/min.to-60~-70℃。 The survival rate of the callus cells was maximal when rewarmed at the rate ofmore than 160℃/min. -20~-40℃ was the major temperature scope of the callus cellinjury;-60~- 75℃was the security temperature scope when callus cells were im-mersed in liquid nitrogen;-60℃ and-75℃ were the upper and lower limits of criticaltemperatures respectively.
【Key words】 Actinidia chinensis; callus cell; super low temperature preservation; liquid nitrogen; germplasm preservation;
- 【文献出处】 西北农业大学学报 ,ACTA UNIVERSITATIS AGRICULTURAE BOREALI-OCCIDENTALIS , 编辑部邮箱 ,1995年02期
- 【分类号】S129
- 【被引频次】13
- 【下载频次】81