【摘要】 用ＲＡＰＤ分析６个农艺性状不同的小麦─黑麦抗条锈病衍生系材料，建立与黑麦抗条锈病基因连锁的分子标记。结果表明，所用６个随机引物中，４个引物在６个衍生系中扩增出６个黑麦特有的产物，其大小约１９７～９８０ｂｐ，分别被标记为Ｊ１７，Ｊ１８ａ，Ｊ１８ｂ，Ｊ１１ａ，Ｊ１１ｂ和Ｄ５。其中，Ｊ１７、Ｊ１１ｂ和Ｄ５标记在６个衍生系中都存在，是与黑麦抗条锈病基因连锁的ＲＡＰＤ标记；Ｊ１１ａ在４个衍生系中存在；Ｊ１８ａ和Ｊ１８ｂ在３个衍生系中存在。表明这６个衍生系可能涉及３～６个黑麦片段的易位，是不同于１Ｂ／１Ｒ类型的一批新的小麦─黑麦异易位系。结果还表明，ＲＡＰＤ分析在小麦遗传背景中检测异源染色体片段转移，是一项有效的技术，有望成为早代选择异易位系的一个分子指标。更多还原

【Abstract】 Detection of rye gene for resistance to stripe rust using RAPD marker was performed using sixwheat-rye derivatives. Four out of skprimers yielded six sPecific products of rye(J17,J18a,J18b,J11a,J11b and D5),0f which J17,J116 and D5 markers have been identified in all of six deriva-tives,J11 a in four derivatives , J18 a and J 18b only in three derivatives.RAPD assay for identify-ing rye or alien DNA fragment in wheat genetic background is suggested as a way to isolate newtranslocation in selection of early generation.Furthermore,in studying on alicn gene,effectof envi-ronment could be induced,breeding procedure and selection efficiency could be improved.更多还原