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河北杨体细胞抗盐性突变体离体筛选的研究

Studies on Selection of Salt-Resistant Mutants ofPopulus hopeiensis by Means of Somatis Culture in Vitro

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【作者】 李周岐周志华郭军战刘西平毕春霞

【Author】 Li Zhouqi; Zhou Zhihua Guo Junzhan;Liu Xiping; Bi Chunxia(Dept. of Foreslry, NWFC, 712100, Yangling Shaanxi)

【机构】 西北林学院林学系

【摘要】 以河北杨优树无性系一年生枝条为外植体诱导愈伤组织,建立了河北杨体细胞抗盐性突变体离休筛选的实验系统,并以NaCl为选择剂,从未经诱变处理及 ̄(60)Coγ-射线或EMS诱变处理的愈伤组织材料中,通过一步和多步选择程序进行了抗性愈伤组织的筛选。共得到7个抗性变异系,并均已分化出完整植株。

【Abstract】 One-year-old twing of plus tree’s clone of Populus hopeiensis was used as the explantto induce callus. The experiment system to selsct salt-resistant mutants of P. hopeiesis was establishedby means of somatic culure in vitro. Using NaCl as selection agent, the mutants have beenseperated through the methods of one-step selection and poly-step selectiom from the callustreated either by  ̄(60)Co γ-rays or EMS and the callus with no treatmrnt. 7 resistant variation lineshave been chosen and all of them have regenerated new plants.

【基金】 国家"七五"攻关项目
  • 【文献出处】 西北林学院学报 ,JOURNAL OF NORTHWEST FORESTRY COLLEGE , 编辑部邮箱 ,1995年03期
  • 【分类号】S792.119
  • 【被引频次】28
  • 【下载频次】80
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