【摘要】 与链霉菌分化有关的whiG结构基因已亚克隆到链霉菌表达载体pAK203的tipA启动子下游.该启动子能被硫链丝菌素诱导.whiG基因的诱导表达不仅可使天蓝色链霉菌孢子形成缺陷突变株C71恢复产生孢子的能力,而且也能使天蓝色链霉菌野生型菌株J1501和变铅青链霉菌TK54的孢子形成更为丰满.Western杂交也进一步证实了诱导表达后whiG基因产物——σ^whiG产量的增加.这为依赖于σ^whiGRNA多聚酶的发育调控启动子体外转录的研究提供了有利条件.更多还原

【Abstract】 whiG gene has been subcloned into Streptomyces expression vector pAK203containing inducible promoter tipA. The expression of whiG gene promoted the sporeformation of S. coelicolor J1501 and recovered the sporulation ability of whiG-de-ficient S. coelicolor C71. Increased amount of whiG gene product was detected byWestern blot hybridization after induction of thiostrepton. It will be helpful for thefuture study of in vitro transcription of whiG-dependent promoters.更多还原