【摘要】 烟色红曲霉(Monacus fulginosus)M-101菌株经麦麸固态培养生成耐热脂肪酶和酯酶。产酶的适宜条件为:培养温度30℃,初始pH 3.0—3.5,麸曲初始含水量75％。培养4—5天后,脂肪酶活力可达207u/g,酯酶活力达14.6u/g。粗酶试验表明,脂肪酶和酯酶的最适反应温度为50℃,脂肪酶最适反应pH为6.0。酯酶最适反应pH为6.8。酯酶耐热性略高于脂肪酶,在55℃处理1小时和45℃处理24小时,两种酶活力基本不变。更多还原

【Abstract】 Thermostable lipase and esterase were produced by Monascus fulginosus strain M-101 in solid-state fermentation. The optimal conditions for enzyme production were incubation temperature of 30℃, initial pH value of 3.0 to 5.5, moisture level of 75% and incubation time of 4 to 5 days. The yield of lipase and esterase was 207 u/g and 14. 6 u/g. respectively. Assay of the crude enzyme showed that optimal temperature for lipase and esterase was 50℃ , but maximum activity of lipase occured at pH 6.0 and esterase at pH 6. 8. Both enzymes almost retained 100% activity after treated at 55℃ for 1 hour and 45℃ for 24 hours.更多还原