【摘要】 研究了不同ｐＨ条件（４．５～６．８）对钙离子荧光指示剂Ｉｎｄｏ－１－Ｋ＋及Ｆｕｒａ－２ＡＭ导入绿豆下胚轴原生质体的影响．结果表明，在正常生理条件下（ｐＨＳ．６），有４０％以上的原生质体被标记；在将Ｉｎｄｏ－１－Ｋ＋和Ｆｕｒａ－２ＡＭ导入绿豆下胚轴、胡萝卜根韧皮部、蚕豆叶肉、小白菜下胚轴、水稻花药愈伤组织和鸢尾花粉等细胞原生质体的研究中，发现不同材料之间具有明显的差异．更多还原

【Abstract】 Different pH conditions (4. 5～6. 8) affecting indo-1--K+ or fura-2 AM loading into Phaseolus radiatus hypocotyl protoplasts have been investigated,through 1. 5～2. 0hours incubation at pH 5. 6,more than forty percent of the protoplasts can be loaded. On the same conditions,protoplasts of Daucus carota root phloem,Brassica chinensis hypocotyl, Phaseolus radiatus hypocotyl, Viola faba mesophyll,Iris tectorum pollen and Oryza sativa anther callus show diffent loaded ratio after incubated with indo-1-K+ or fura-2 AM.更多还原