【摘要】 本文报告利用ＲＰ－ＨＰＬＣ与紫外检测器，以吡哌酸（ＰＰＡ）为内标，快速测定人血清中依诺沙星（Ｅｎｏｘａｃｉｎ，ＥＮＸ）浓度的方法。作者采用Ｕｌｔｙａｓｐｈｅｒｅ－ＯＤＳ分析柱，以０．１ｍｏｌ／Ｌ柠檬酸溶液（ｐＨ＝２．５）－甲醇－乙腈（７：２：１）为流动相，波长选择２６５ｎｍ，以内标法定量。实践表明本方法具有灵敏、快速、准确等特点，适用于依诺沙星血药浓度的监测及临床药理学研究。更多还原

【Abstract】 A rapid method for the reversed-phase high performance liquid chromatography using pipemedic acid as the internal standard was established.The method uses a 265 nm wavelength ultraviolet detector and reversed-phase ultrasphere-ODS(5μm,250 ×4.6mm)column,0.1m citric acid-methanol-acetonitril(7:2:1)as mobile phase.The detetor was operated at 0.2 AUFS.The retention times for pipemedic acid and Enoxacin were 4.4 9min and 5.74min respectively. Good linearity was obtained in the concentration range of 1-50μg/ml,with standard calibration curve y=12.0118x+0.1722 and the correlation coefficient r=0.9996.The detection limit was 0.5μg/ml.更多还原