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分光光度法直接测定鸡蛋中的锌

DIRECT DETERMINATION OF Zn IN EGGS BY SPECTROPHOTOMETRY

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【作者】 张阅军李湘李晓亮王伟程原生

【Author】 Zhang Yuejun et al.(Analysis and Test Center of Shanxi Agricultural University, Taigu, 030801)

【机构】 山西农业大学测试中心

【摘要】 本文首次使用适量2moi/LHCI,使鸡蛋中的锌元素与蛋白质分离,用10%的三氯乙酸沉演蛋白质,经离心得透明上清液。锌试剂(Zincon)作显色剂。直接用分光光度法测定鸡蛋中的Zn(2+)含量,同时用干灰化法和湿消化法制各样品,原子吸收法测定Zn(2+)含量,比较结果一致。

【Abstract】 In this paper, a method of direct determination of Zn in eggs by spectroptometry with Zincon as a color reagent has been studied. Zn were separated from protein by using hydrochloric acid (2mol/L), and protein was precipitated by using trichloroacetic acid (10% ). The determination was implemented by introducing the solution after centrifugalizing. The values determinated by the presented method are very good in agreement with the results obtained by using dry-ashing and wet-digesting atomic absorption spectrophotometry.

【关键词】 分光光度法直接测定
【Key words】 SpectrophotometryDirect determination of Zn
  • 【文献出处】 山西农业大学学报 ,JOURNAL OF SHANXI AGRICULTURAL UNIVERSITY , 编辑部邮箱 ,1995年01期
  • 【分类号】S852.1
  • 【下载频次】141
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