【摘要】 当胃蛋白酶与猪脾转移因子在体外模拟胃酸（pH2．0）的情况下，于37℃水浴中作用1h及2h后，其紫外（A254nm）吸收值及对经45℃处理1h的T淋巴细胞EaRFC和EtRFC形成率与对照PS－TF之间均无显著差异（p＞0．05）。200nm～300nm扫描曲线表明，PS－TF经胃蛋白酶作用2h后，在230nm以下主要由肽键形成的吸收峰明显下降。表明胃蛋白酶对PS－TF中多肽链某些部分的水解与PS－TF的生物活性无关。更多还原

【Abstract】 After porcine spleen transfer (PS-TF) was treated with pepsin at pH2．0,37℃ for 1 and 2 hours, the A254nm and T lymphocyte (after being treated at 45℃ for 1 hour) EaRFC and EtRFC formation rate of it showed no significant difference compared with control (p＞0．05). Scanning curve diagram at 200~300nm showed that the absorption peak of petide bond at below 230nm were significantly dropped after PS-TF were treated by pepsin for 2 hours. It proved that the biological activity of PS-TF could not be influenced by the hydrolysis of some polypeptide chains in it with pepsin.更多还原