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1,6-二磷酸果糖产生菌的筛选及最佳培养条件的研究

Screening Strains Producing FDP and Selecting Optimal Incubation Conditions

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【作者】 胡永红应汉杰万红贵何永祥欧阳平凯

【Author】 Hu Yonghong; Yin Hanke; Wan Honggui;He Yongxiang; Ouyang Pingkai(Nanjina Institute of Chemical Technology, Department of Biotechnolongy and Bioengineering, Nanjing)

【机构】 南京化工学院生物工程与科学系

【摘要】 1,6-二磷酸果糖(FDP)广泛应用于治疗心血管疾病.本文进行了1,6-二磷酸果糖产生菌的筛选和最佳条件的研究。筛选出菌株HY2具有较高的产1,6-二磷酸果糖酶活力,高达160mgFDP/gwetcells,菌体生长的最适培养条件为:麦芽汁培养基糖度为12柏林,培养温度为28℃,pH6.0~6.5,菌体收率达7.0%左右。

【Abstract】 FDP has been extensively used in treating cardiovascular diease. In this paper wescreened FDP producting strains and we also studied the optimal in cubation conditions. Agony the strains tested, the best one was strain HYZ whose enzyme activity was about160mg FDP/g wet cells. The optimal incubation conditions were malt extract medium 12Brix, PH6. 0-6. 5, cultural tdemperature 28℃ and the yield of strains wus bout 7%.

【关键词】 酵母1.6-二磷酸果糖菌株筛选培养条件
【Key words】 Yeast FDPStrainsSelectionIncubation condition
  • 【分类号】TQ460.38
  • 【被引频次】4
  • 【下载频次】83
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