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复合四倍体异育银鲫的血红蛋白和红细胞的同工酶分析

AN ANALYSIS OF HAEMOGLOBIN AND ISOZYMES IN THE ERYTHROCYTE OF MULTIPLE TETRAPLOID ALLOGYNOGENETIC CRUCIAN CARP

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【作者】 朱篮菲桂建芳梁绍昌蒋一

【Author】 Zhu Lanfei; Gui Jianfang; Liang Shaochang and Jiang Yigui(Institute of Hydrobioloty.the Chinesc Acadcmy of Sciences.Wuhan 430072)

【机构】 中国科学院水生生物研究所

【摘要】 用聚丙烯酰胺梯度凝胶电泳,分析了银鲫、复会四倍体异育银鲫和红鲤的血红蛋白及红细胞中的4种不同的同工酶(MDH、LDH、EST、SOD)。结果表明复合四倍体异育银鲫的一些座位(血红蛋白和EST、SOD),除了银鲫的基因表达外还观察到红鲤基因的表达,出现了银鲫原来所没有的区带或原有的区带受到抑制等现象,显示出复合四倍体异育银鲫具有类似于杂种的遗传性伏,另一方面从4种不同的同工酶表型分析,表明复合四倍体异育银鲫的同工酶基因表达较接近银鲫。

【Abstract】 The haemoglobin and four different isozymes were analysed in erythrocytes ofsilver crucian carp, Carassius auratus gibclio and red common carp,Cyprinus car-pio red variety,and their hybrid,the multiple tetraploid allogynogenetic cruciancarp,C. auratus,using polyacrylalmide gradient gel electrophoresis technique。Someloci(Hb、EST、SOD)in multiple tetraploid allogynogenetic crucian carp expressednot only the maternal but also the paternal genes.Some bands appeared which isnot original in silver crucian carp,and some original bands in silver crucian carpwere repressed.It is thus indicated that the multiple tetraploid allogynogeneticcrucian carp exhibited features of a hybrid,and that isozyme gene expressions ofthe allogynogenetic crucian carp should be much closer to the silver crucian carpfrom the analyses of phenotypes of four different isozymes。

【基金】 中国科学院院长基金
  • 【文献出处】 水生生物学报 ,ACTA HYDROBIOLOGICA SINICA , 编辑部邮箱 ,1995年01期
  • 【分类号】Q959.468
  • 【被引频次】11
  • 【下载频次】78
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