【摘要】 <正> 哺乳动物细胞的大规模培养是生产许多医学上重要生物制品的一种主要方法之一。很多有工业价值的动物细胞都是贴壁细胞,必须附着在一定的表面上才能生长,微载体培养是一种有效的体系。用于微载体培养的反应器多为搅拌式反应器,近年来,流化床式反应器越来越引起生化工程学家的重视。有希望用于大规模动物细胞培养的主要是液升和气升式。液升式反应器的优点是培养液可以间接氧饱和,气泡和细胞不直接接触。在气升式流态反应器中,气泡与细胞直接接触,其优点是操作方便,设备简单。本文报道通过气升流态化反应器实现高密度贴壁细胞培养工艺条件的研究。更多还原

【Abstract】 A 200ml air-lift bioreactor was designed for microcarrier cultrue according to its characteristics. Vero cells were cultivated on microcarriers in the designed reactor. It was found that sparging could induce cell injury in the culture, and cells could be protected by supplementing the medium with 0. 3% Pluronic F-68, resulting in significant increasing the final cell density. There was no different effect on cell growth in the adopted ventilation range , less than or eqaul to 0. 12vvm. The final cell density could reach 1. 13×106/ml using 2g/L Cytodex 1 under appropriate conditions in this study. A contact-inhibited growth model was established to describe the growth kinetics for anchorage-dependent cells.更多还原