【摘要】 采用固定化青霉素酰化酶(Penicillin acylase)在反应器中进行青霉素G水解生产6-APA,同时与离子交换柱相组合以连续地去除反应混合液中的苯乙酸。建立了离子交换柱的分格模型(Compartment model),在确定了青霉素G和苯乙酸沿柱高的浓度分布的基础上,与描述固定化酶反应器的状态方程相结合,得到了固定化酶-离子交换组合系统的数学模型。在将计算机模拟值与实验值进行验证后,探讨了组合系统中树脂量、循环流速和组合起始时间对青霉素G酶解过程的影响。更多还原

【Abstract】 A composite system of an immobilized enzyme reactor combined with an ion exchange column was employed for hydrolysis of PenG to 6-APA with continuous removal of PAA. In this study , a mathematical model of PenG hydrolysis to 6-APA in the composite system was developed based on the compartment model, the profile of concentration in the ion exchange column and the enzyme kinetics in the immobilized enzyme reactor. After checking the simulation valuse and experimental data, the effects of the resin volume, the flow rate and the switching time on the time required to reach the desired conversion rate was also examined by computer simulation.更多还原