【摘要】 用杂交瘤技术制备了5株产生抗黄曲霉毒素B₁单克隆抗体的杂交瘤细胞株。对其中之一AFB₁-2H8进行了较系统的研究。AFB₁-2H8属IgC₃。纯化腹水抗体效价约5×10⁶。ELISA检测标准毒素的线性范围为0.5～50ng/ml。最低检出量为0.01ng/ml。该单抗与参试的其它黄曲霉代谢物的交叉反应系数为0～0.21,该抗体有较大的应用价值。更多还原

【Abstract】 Five hybridoma cell lines producing monoclonal antibodies (McAb) against aflatoxin B1 (AFB1) were established after fusion of mouse myecloma cells (Sp-2/0-Ag-14) with spleen cells isolated from male BALB/c mice immunized with AFB1-BSA conjugate. Among them, a McAb which was designated AFB1-2H8 was of the subtype IgG3 and the ascitic fluid of it gave suitably high dilution titres (1: 5X106). The sensitivity of a competitive enzyme- (linked immunosorbent assay (ELISA) using AFB1-2H8 for AFB1 was demonstrated that the linear range was 0. 5~50ng/ml and the minimum detectable concentration of AFB1 was 0. 01ng/ml. The specificity of the McAb was determined and it was shown no cross-rection significantly with any of the metabolites tested. So the McAb and the ELISA described may prove of use in the detection of AFB] foods and feeds.更多还原