【摘要】 以甜菜坏死黄卧病毒（BNYVV）内蒙分离物的总RNA为模板,通过反转录和PCR扩增获得75kDa通读蛋白基因54kDa片段的目的片段。将其克隆到pGEM-7Zf（+）上并转化DH5α得到了含有完整54kDa片段的重组子pGBW52。采用双脱氧终止法进行序列分析。结果表明内蒙分离物的54kDa。片段全长为1509nt,与法国的F₁₃分离物相比缺失了3个核苷酸。其核苷酸序列和由此推导的氨基酸序列的同源性分别为94.97％和96.42％。更多还原

【Abstract】 The RNAs was extracted from purified beet necrotic yellow vein virus (BNYVV) isolated from Inner Mongolia of China. The first strand of cDNA encoding 54kDa fragment of 75kDa readthrough protein was synthesized from viral RNA template using reverse transcription, and 1. 5kb fragment was obtained after 30 PCR amplification cycles. The restriction map of the target fragment cloned into pGEM-7Zf(+) and its whole sequence has been analyzed. The result shows 54kDa fragment of 75kDa readthrough protein gene of BNYVV isolated from Inner Mongolia of China has 1509nts. Comparaing with published F13 isolate,this fragment was deleted with 3nts and shares 94. 97% and 96- 42% homology in terms of nucleotide sequence and deduced amino acid sequence respectively.更多还原