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枸杞子糖蛋白的分离纯化、物化性质及糖肽键特征
Isolation,Purification and Properties of LbGP and Characterization of Its Glycan-Peptide Bond
【摘要】 从宁夏枸杞子提取得到的粗多糖,经DEAE-Cellulose和SephadexG-100柱层析,得到均一的枸杞子糖蛋白LbGP。分子量由SDS-PAGE测定为88kd,糖含量为70%,糖组成为Ara:Gal:Glc=2.5:1.0:1.0(摩尔比),并含有其他18种天然氨基酸。初步分析表明LbGP是O-连接的糖蛋白。
【Abstract】 An immunoaotive glycoprotein(LbGP) was isolated from the fruit of Lycium barbarum L..Its MW was 88 kDa as determined by SDS-PAGE. Its carbohydrate content was up to 70%.Component analysis showed that it was composed of Ara,Gal and Glc in a molar ratio of 2.5:1.0:1.0,and 18 kinds of amino acids.The linkage between the glycan and the core protein backbone may be O-linkage.
- 【文献出处】 生物化学与生物物理学报 ,ACTA BIOCHIMICA ET BIOPHYSICA SINICA , 编辑部邮箱 ,1995年02期
- 【分类号】Q949.777.7
- 【被引频次】180
- 【下载频次】1069