【摘要】 用牛血清白蛋白－戊二醛交联剂把葡萄糖氧化酶固定在Ｎａｆｉｏｎ－二茂铁修饰电极上，最后在电极上修饰一层Ｎａｆｉｏｎ膜，制备成葡萄搪传感器，电活性物质如抗坏血酸、尿酸等对葡萄糖的测定无干扰。该传感器的线性范围为５．０×１０￣（－４）－１．３×１０￣（－２）ｍｏｌ／Ｌ，响应时间小于６０ｓ．更多还原

【Abstract】 Electrical communication betweenthe flavin adenine dinucleotide redox centersof glucose oxidase and glassy carbon electrodehas been achieved by employing ferrocene im-moblized in Nafion membrane,The glucosesensor is made by using bovine serum albuminand glutaraldehyde to immobilize glucose oxi-dase on Nafion-ferrocene modified electrode.It is further coated by Nafion,which is aque-ous solution made from Nafion methanol solu-tion by distilling and is not detrimental to theenzyme but to forstall electrochemically active anionic interferents such as ascorbate andurate from reaching the electrode.The Poly-mer coating also avoids ferrocene′s leakingand keeps the biosensor from fouling agents。The biosensor responds rapidly to glucose inless than 60 second and its calibration linearcurve is from 5.0× 10￣(-4)to 1.3× 10￣(-2)mol/L.更多还原