【摘要】 以中国对虾Ｐｅｎａｅｕｓｃｈｉｎｅｎｓｉｓ虾头经处理，得到虾头透明质酸酶酶液，再经沉淀、硫酸铵反抽提、ＤＥＡＥ－纤维素提取和ＳｅｐｈａｄｅｘＧ－７５凝胶层析。纯化倍数达７２０倍，比活８４０ｕ·ｍｇ－１蛋白。该酶最适ｐＨ为４．５，最适温度为５０℃。更多还原

【Abstract】 Hyaluronidase from shrimp head was isolated and characterized.The enzyme was purified to 720 fold by precipitation, ammonium sulfate backfractionaton,DEAEcellulose extraction and Sephadex G-75 gel chromatography.Specific activity was 840 u·mg-1 protein. Optimum pH is at 4. 5,and optimum temperature at 50℃.更多还原