【摘要】 在现场条件下,用双盲法对单克隆抗体反向间接血凝试验(McAb—RIHA)诊断血吸虫病的效果进行了观察。结果显示,McAb—RIHA 与 Kato 法粪检总的样本检测符合率达80.0%,与粪检阳证样本的符合率(校正)达83.9%,对粪检阳性,McAb-RIHA 阴性的样本复查排除37.5%(3/8)的粪检假阳性,对粪检阴性,McAb—RIHA 阳性的样本Kato 法复查一次(3张涂片)检出24.6%的样本阳性(14/57),是总样本粪阳率6.79%(34/501)的2.62倍,与单克隆抗体斑点酶联免疫吸附试验(McAb—Dot—ELISA)的检测结果比较,两法无显著性差异。实验结果表明,McAb-RIHA 在现场用于诊断血吸虫病具有较为理想的准确性,且其操作简便、诊断快速,具有较高的现场实用性。更多还原

【Abstract】 It was studied to diagnose schistosomiasis using McAb-RIHA with the monoclonal antibody in double-blind in a feild.The results showed that the coincidence rate between MEAD-RIHA and Kato-Katz for all of identified samples was 80% and the coincidence rate of positives between McAb-RIHA and stool examination was 83.9% (check rate),35% (3/8 cases) false positives in positives by stool examination but negatives by McAb-RIHA was evacuated.24% to cases of negatives by stool examination but positives by McAb-RIHA,which is 2.62 times of the positive rate by stool examination for all of the samples, wasidentified as positives by Kato-Katz(3 smears)once again.It was same between the results by McAb-RIHA and by McAb-Dot-ELISA after comparation,there is no significant difference between two diagnostic methods.The studied result showed that McAb-RIEA is more satisfactory accuracy for diagnosis of schistosomiasis and simple in operation and faster in diagnosis.The diagnostic method is more practical in feid.更多还原