【摘要】 介绍了载脂蛋白ＡⅠ的一种分离纯化方法。用离心法制备人血清高密度脂蛋白（ＨＤＬ），乙醇／乙醚（１＇３，Ｖ／Ｖ）脱脂，经ＳｅｐｈａｃｒｙｌＳ－２００凝胶层析及ＤＥＡＥ－５２离子交换梯度洗提得纯化的载脂蛋白（ＡｐｏＡＩ）．ＳＤＳ－聚丙烯酰胺凝胶电泳证明为单一条带。更多还原

【Abstract】 The method of purificating ApoA Ⅰ is presented. Human serum HDL isprepred with supercentrifugation, which is degreased with alcohol/ether(3/1V/V),chro-matogrphyed with Sephacryl S-200 and DEAE-52 to obstain purified ApoA Ⅰ,which isverified to be unique belt by gel electrophosis.更多还原