【摘要】 枯草杆菌菌株Ｂ３（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）用ＮＢ培养液振荡培养（３０℃，１１０ｒ·ｍｉｎ－１ｐｍ，６０ｈ），离心（５０００ｒ·ｍｉｎ－１，１５ｍｉｎ，４℃）去除菌体，上请液经超滤浓缩与离子交换层析，分离并纯化出一种抗植物病原真菌蛋白，经ＨＰＬＣ鉴定纯度为９８．０２２５％。电泳分析该蛋白分子量为１２８８２．５Ｄ，等电点为４．８。氨基酸组成分析含１３种氨基酸残基，其中谷氨酸含量为最高，甘氨酸、半胱氨酸、苯丙氨酸和精氨酸含量则较低。纯化后的抗菌蛋白对小麦赤霉病菌（Ｆｕｓａｒｉｕｍｇｒａｍｉｎｅａｒｕｍ）和小麦纹枯病菌（Ｒｈｉｚｏｃｔｏｎｉａｃｅｒｅａｌｉｓ）有很强的抑菌作用，有效浓度分别为３１．２５，６２．５ｍｇ·ｋｇ－１。更多还原

【Abstract】 The NB culture of B3 was centrifuged and the supernatant wasconcerntrated by ultrafiltra-tion,A kind of antifungal protein was characterized from the concerntrate by DEAE一Sepharose F. F col-umn chromatography, FPLC superose一12 gel filtration and monoQ column chromatography.Its puritywas identified to be 98.0225%by HPLC. The molecular weight and isoelectric point were determined tobe l2 882.5 daItons and 4. 8 respectively by autornated electrophoresis. An1mino acid analysis result indi-cated that it consisted of l3 ammino acids,and the content of Glu ls the hightest. Characterized antifungalprotein was greatly inhibitory to Fusarium graminearum and Rhizoctonia ceieatis,and the effective con-cerntration was 31.25 mg· kg-1 and 62.5mg· kg-1,respectively.更多还原