【摘要】 １７α－羟化酶催化雄激素合成的中间步骤，它对了解睾丸间质细胞内雄激素的合成具有重要的意义。本研究首先提取小鼠睾丸组织ＲＮＡ，然后以１７α－轻化酶ＤＮＡ两端的特定序列为引物，反转录合成ｃＤＮＡ，再以ｃＤＮＡ为模板行ＰＣＲ扩增ｃＤＮＡ。结果显示，反转录ＰＣＲ后获得一条３６０ｂｐ的电泳带，为１７α－羟化酶ｃＤＮＡ。结果表明，用反转录ＰＣＲ方法扩增睾丸１７α－羟化酶ｍＲＮＡ是切实可行的。更多还原

【Abstract】 The synthesis of androgen is catalysised by 17-α-lydroxylase. The study of 17-α-lydroxylase mRNA could improve the understanding of this procedure in Leydig’s cells. In this study,mouse testis RNA was isolated at first. Then specific nucleic sequences at the end of 17-α-lydroxylase DNA were used as primer to synthesis cDNA with 17-α-lydroxylase mRNA as templet; At last,PCR was used to emplify cDNA, The result showed that a 360bp cDNA was obtained after PCR was useful for the empilification of l7-α-lydroxylase mRNA.更多还原