【摘要】 用血小板自动平衡聚集仪建立血小板活化因子的生物活性检测法.测定了20例急性肾炎（AGN）患儿血浆PAF和尿lyso-PAF的水平,同时测定血C₃的含量.结果表明,AGN急性期血浆PAF及尿lyso-PAF显著高于对照组;AGN恢复期血浆PAF及尿lyso-PAF显著高于对照组;AGN恢复期血浆PAF及尿lyso-PAF均降至正常;血C₃与血浆PAF、尿lyso-PAF均呈明显的负相关.提示AGN急性期血PAF、尿lyso-PAF水平的增高与补体的沉积、肾小球的免疫损伤密切相关;PAF在急性肾炎的发病机制中可能具有重要的作用.更多还原

【Abstract】 Using the platelet aggregation machine a bioactivity assay was established to measure the plasma PAF and urine lyso-PAF in 20 children with acute glomerular nephri-tis (AGN). Meanwhile,the serum level of C3 was also measured. The results indicated : (1) the level of both plasma PAF and urine lyso-PAF in acute stage of AGN was higher than that of the control group; (2)the level of plasma PAF and urine lyso-PAF in recovery stage of AGN returned to normal level ;(3)the level of serum C3 was negatively related to the level of plasma PAF and urine lyso-PAF. These nesuets suggested that the increase of plasma PAF and urine lyso-PAF was related to both complement deposation and glomerular immune defi-ciency. PAF might play an important role in the pathogenesis of AGN.更多还原