【摘要】 探讨了邻苯三酚－Ｈ２Ｏ２发光系统的最适实验条件。结果表明，发光溶液的最适ｐＨ为６．５～７．０，邻苯三酚浓度为０．３％，Ｈ２Ｏ２浓度为０．４５％，测定前需３７℃暗适应１５ｍｉｎ。发光强度用ＤＧ３０３０发光仪检测。该法测定人型支原体抗原的敏感度为１．０２ｎｇ／ｍｌ。用本法与ＥＬＩＳＡ对５１份临床阴道分泌物标本平行测定，结果没有显著差异。更多还原

【Abstract】 uminescant Enzyme Immunoassay(LEIA)was established for detection of Mycoplasma ho-minis antigen with HRP-Pyrogallol-H2O2 system,It is found that pH 6.5～7.0,0. 3%pyrogallol,0.45%H2O2 are suitale conditions for the assay.Incubation under 37℃ for 15 minutes before test-ing chemiluminescence value is necessary for stablizing the results. The sensitivity for M. hominisantigen is 1.02 ng /ml.Results of 51 clinical specimens with LEIA were not significantly differentfrom those with ELISA. It is suggested that LEIA be a usuful method for epidemiologic study ofMycoplasma hominis infection as well as ELISA.更多还原